This HT-free formulation (without hypoxanthine and thymidine) has been specifically designed for applications involving DHFR-based selection and amplification systems, particularly with CHO-DG44 cell lines. The absence of HT allows efficient selective pressure during clone selection and maintenance while supporting strong productivity and stable culture performance. Suitable for fed-batch production processes, ForCHO® Medium without HT can be used together with ForCHO® Feed Alpha and Feed Beta to achieve high recombinant protein yields in demanding biomanufacturing applications.
This product corresponds to the medium previously marketed as First CHOice®.
• No change in formulation
• No change in raw materials
• No change in performance
• Manufactured under InnoBioMil quality standards
Frequently asked questions
ForCHO® Medium without HT is designed for CHO cell culture in recombinant protein production, particularly for applications involving DHFR-based selection and amplification systems such as CHO-DG44 workflows.
The absence of hypoxanthine and thymidine (HT) enables efficient selective pressure during clone selection, amplification, and maintenance while supporting stable cell culture performance.
Yes. The medium is optimized to support robust cell growth, high viability, and strong recombinant protein productivity while maintaining stable culture performance.
Yes. ForCHO® Medium without HT is fully compatible with ForCHO® Feed Alpha and ForCHO® Feed Beta for fed-batch culture applications.
Yes. ForCHO® Medium without HT is the direct continuation of First CHOice® Medium without HT, with no change in formulation or performance.
Following acquisition and integration, the product is now part of the ForCHO® range by InnoBioMil.
Yes. The product is designed to ensure full continuity for existing applications and processes.



